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KMID : 0366919970090010061
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Sungkyun Pharmceutical Journal
1997 Volume.9 No. 1 p.61 ~ p.66
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Inhibition of benzo[a]pyrene-DNA adduct formation by Aloe barbadensis Miller
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Kim Hyung-Sik
Lee Byung-Mu
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Abstract
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The antigenotoxic and chemopreventive effect of Aloe barbadensis Miller (polysaccharide fraction) on benzo[a]-pyrene (B[a]P)-DNA adducts was investigated in vitro and in vivo. Aloe showed a time-course and dose-dependent inhibition of [^3H]B[a]P-DNA adduct formation in primary rat hepatocytes (1×10^6 cells/ml) treated with [^3H]B[a]P(4 nmol/ml). At concentrations of 0.4-250 μg/ml aloe, the binding of [^3H]B[a]P metabolites to rat hepatocyte DNA was inhibited by 9.1-47.9%. Also, in rat hepatocytes cultured for 3-48 h with aloe (250 μg/ml) and [^3H]B[a]P (4 nmol/ml), [^3H]B[a]P-DNA adducts were significantly reduced by 36% compared with [^3H]B[a]P alone. Aloe also inhibited cellular uptake of [^3H]B[a]P in a dose-dependent manner at a concentration of 0.4-250 μg/ml by 6.3-34.1%. After a single oral administration of B[a]P to male ICR mice(10 mg/mouse), benzo[a]pyrene diol epoxide I (BPDE-I)-DNA adduct formation and persistence for 16 days following daily treatment with aloe (50 mg/mouse) were quantitated by enzyme-linked immunosorbent assay using monoclonal antibody 8E11. In this animal model, BPDE-I-DNA adduct formation was significantly inhibited in various organs (liver, kidney, forestomach and lung) (P＜0.001). When mice were pretreated with aloe for 16 days before B[a]P treatment, inhibition of BPDE-I-DNA adduct formation and persistence was enhanced. Glutathione Stransferase activity was slightly increased in the liver but cytochrome P450 content was not affected by aloe. These results suggest that the inhibitory effect of aloe on BPDE-I-DNA adduct formation might have a chemopreventive effect by inhibition of B[a]P absorption.
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